Restoration of Enzyme Activity Using Heat Shock Proteins

Restoration of enzyme activity using hot pants stupor proteins

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The highly conserved heat shock proteins (HSPs) ar ATP-binding proteins that go away as molecular chaperones which facilitate the synthesis and folding of proteins. In addition, HSP plays a major role in protecting the carrellphone against heat or other forms of filter. The HSP family consists of many proteins. According to their molecular weight, heat shock proteins are divided into 5 families. For example, hsp100, hsp90, hsp70, hsp60 and about low molecular weight HSPs (Vierling lab, 2000). Among them, the intracellular hsp70 is a oecumenical marker of stress protein whose expression are increased by different cell stresses, such as heat, exposure of the cell to toxins, metabolically harmful substances and complex metabolic processes. Furthermore, the hsp70 play a significant role in the cells machinery for protein binding, and help to protect cells from stress. The hsp70 functions open been subject area in ocular tissues, but the exact functions are still uncertain (Oc et al., 2008).

It has been known that several heat shock proteins act as chaperones (assist protein binding) or ATP-dependent proteases.



For instance, Dnak is the prokaryotic analogue of eucaryotic Hsp70, and combine with DnaJ and GrpE heat shock proteins, which constitute a cellular chaperone system for protein binding in prokaryotes (Huang and Lee, 2010). The synthesis of heat shock protein is induced when the environment of an organism physiological is in a bad way(p) and proteins are denatured. However, this introduces the question whether other proteins with ATP can play the same role as hsp70.

Thus the present study aimed to investigate Hsp70 variation in response to heat stress in mammalian, to test the ability of an Hsp70 to restore the activity of a heated-inactivated enzyme, lactate dehydrogenase [LDH], in addition, to assess whether ATP can enchant this activity.



Methodology
To carry out the experiment restoration...If you want to get a full essay, order it on our website: Ordercustompaper.com



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